If proper maintenance is not performed on the wastewater treatment plant, the first issue to occur will be over-sludging. This manifests as an increase in the concentration of activated sludge in the activation process beyond the optimum (3-4 kg/m3, which corresponds to about 30% of the volume of the sample taken after settling for 30 minutes). As a result, activated sludge will eventually start to escape into the outflow, typically occurring when the sludge concentration exceeds about 60-70%.